avatar

JonathanT

Last updated: Jul 16, 2020

pET22bTraVN

TraVN (N. gonorrhoeae MS11 NCBI:CP003909) and pET22b were PCR amplified using primer pair 67/68 and primer pair 65/66 respectively. The PCR products were combined using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs.).

Draft
Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology. The backbone (pET22b) comes from Novagen.

#
Strain data
Barcode
v1.0 2018ec

2018ec4d95dc442b91e46eb8d1d88f3bb2c707f70

Plasmids

This plasmid was created in this work from pET22b

pET22b: Protein expression vector. AmpR (Novagen)

Ampicillin resistance

Devonshire Building, Newcastle University. ICOS -80 room.