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JonathanT

Last updated: Jul 17, 2020

pKTM25TraVF

TraVF was PCR amplified using primer pair 59/60 and cloned into pKTM25

Draft
Whole genome sequence

This plasmid was constructed in this work: Protein interactions within and between two F-type type IV secretion systems. Birgit Koch, Melanie M. Callaghan, Jonathan Tellechea Luzardo, Ami Y. Seeger, Joseph P. Dillard, and Natalio Krasnogor. 2020. Submitted to Molecular Microbiology.

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Strain data
Barcode
v1.0 e43fe4

e43fe41e38844403bd0064f6346e5dcbb2c660a66

Plasmids

This plasmid was propagated using 10-beta strain: Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14-  ϕ80dlacZΔM15  recA1 relA1 endA1 nupG  rpsL 

This plasmid was created in this work from pKTM25

This plasmid is based on pKTM25 (this study): Bacterial two-hybrid vector designed to express a given polypeptide fused in frame at its N-terminal end with TM domain 1 of E. coli oppB and T25 fragment, p15 ori, KmR The TM region was PCR amplified from pSTM25 with primer pair 1/2 cut with SalI/EcoRI and cloned into SalI/EcoRI digested pKT25.

Kanamycin resistant

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