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JonathanT

Last updated: Aug 04, 2021

BW25113 - BC383

This is the barcoded version of BW25113 using lambda-red as barcoding method (Datsenko, KA and Wanner, BL 2000). CamR was removed by FLP expression (pCP20).

Commited
Whole genome sequence

Datsenko, KA and Wanner, BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97:6640-5

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Strain data
Barcode
v1.0 8328b5

8328b553bfd349f098d19ce9d95e05d6b2c0ad6eb

v1.0 ⎆GTCGTG

Genosignature: GTCGTGTCTGTCAATATCGGGGATAATAAATGTAGATAATGATTTCGTCAAGTCCTTACCCCGACTATACAACAGCAATCCATAGTAATCTGTGCGAATGGTTTAGTAGGACATAACACAAAT

Show GC content diagram

Escherichia coli

ATCC 11775

s284

from parent

rrnB3 ΔlacZ4787 hsdR514 Δ(araBAD)567 Δ(rhaBAD)568 rph-1. BC383 (in between pyrH and frr)

Newcastle University, Devonshire Building. ICOS -80 room.

Mar 01, 2019